what helped - at least 90 % for me was some instructions I had received from the motu support, not sure I can attach them here... So, I dare to past it all here... For me the manual driver de- and reinstall (at the very end) was most effective.
MOTU Pro Audio interface troubleshooting macOS
1.) Reset- in rare circumstances, it’s possible for the unit to
become “wedged” or “frozen”. Resetting the unit will often resolve
the issue. To learn how to reset your unit, visit
http://motu.com/
techsupport/technotes/reset. A rest will delete any custom
configurations or presets. Be sure to export any custom preset
files to your computer before the reset.
2.) Cable- a bad cable can cause a number of unusual problems.
Be sure to test multiple USB and/or Thunderbolt cables.
Additionally, you will want to test connections to all USB or
Thunderbolt ports on the computer.
3.) USB Hub- USB hubs are notorious for causing connection
issues. If you must use a hub, we recommend using a powered
hub. When troubleshooting, make sure to take the hub out of the
signal path and connect directly to the computer’s USB port. The
same principal applies when daisy chaining via a Thunderbolt
dock. Remove any additional Thunderbolt devices when
troubleshooting.
4.) Driver- make sure you are running the latest version of the
MOTU Pro Audio driver for macOS. The installer is available on
motu.com/downloads. Be sure to restart your computer after the
installation process has finished.
5.) Firmware- the web control app will notify you when a new
update for your device is available. MOTU Pro Audio interfaces
have a fail-safe mechanism that will prevent damage to the unit in
the event of a power disconnect during the firmware update
process. It is extremely unlikely that an unsuccessful firmware
update will cause the unit to fail. Make sure to connect the unit to
the computer or router via ethernet to update the firmware. For
the UltraLite mk4 and 828es, the firmware can be updated via
USB. We recommend staying up to date with our latest updates.
6.) macOS High Sierra- the macOS High Sierra update adds an
extra layer of system security. Third party drivers now require
manual activation. If you are running macOS High Sierra, be sure
to open the Security and Privacy page in System Preferences and
click “allow” for any MOTU drivers you wish to use.
Additional suggestions:
7.) Web app access- if you experience trouble accessing the web
control app, open your browser and type ‘localhost:1280’ in the
address bar at the top of the window. This will open the web
control app for any devices that are locally connected to the
computer via USB or Thunderbolt. If your device is connected via
ethernet, type in the unit’s IP address in the address bar. The IP
address for the interface will be displayed on the front panel LCD
when pressing the “ID” button on the front panel.
8.) USB class compliant mode - your MOTU Pro Audio interface
will function via the class compliant USB driver that is built into
macOS and iOS. If you’ve installed the MOTU Pro Audio Driver,
you can force the unit to operate via USB class compliant mode.
To do so, power off the Pro Audio interface, find the Extensions
folder (System HD/Library/ Extensions) and drag the
MOTUProAudio.kext file to the trash. Restart the computer and
then power on the interface. To use the MOTU Pro Audio driver,
simply run the Pro Audio installer available on motu.com. Note
that USB class complaint mode is not possible via Thunderbolt.
9.) Digidesign Drivers- old Digidesign or AVID audio drivers
have been known to cause conflicts with our Pro Audio driver. Be
sure to remove any dated Digidesign drivers if you suspect a
driver conflict. Go to the Extensions folder (System HD/Library/
Extensions) and delete any files with the .kext extension that
contain the word “Digidesign”.
10.) Refresh Preferences- to restore the Discovery App’s
preferences, quit the Discovery App and go to the User Library
folder (hold the Option Key while clicking on the Finder's "GO"
drop-down menu, select Library, then open the Preferences
folder). Find the file named “com.motu.MOTUDiscovery.plist” and
drag it to the trash.
11.) Manual driver remove- to manually remove the Pro Audio
driver, trash the following files:
Macintosh HD/Applications/: MOTU Discovery.app Macintosh HD/
Applications/: MOTU Audio Tools.app
Macintosh HD/Library/Application Support/MOTU/: ProAudio
Macintosh HD/Library/Extensions/: MOTUProAudio.kext
Macintosh HD/Library/Audio/MIDI Drivers/:
MOTUProAudioMIDIDriver.plugin Macintosh HD/Library/Audio/
Plug-Ins/HAL/: MOTUPROAudioServerPlugin.driver Macintosh
HD/ Library/LaunchDaemons/:
com.motu.proaudio.HTTPServer.launchd.plist