Here are a few of mine:
Don't assign Key assignments to numbers. Sometimes you will be turning a knob with the mouse, and pounding on your digits only to discover you suddenly have a curser spewing out random digits!
You can hold shift while doing an assignment for extra use of the same key. This actually can resolve the previous issue. You can do a shift+assign for the number row of keys!
F1-F8 already mute/unmute channels 1-8. Getting to know the use of all the F keys can really come in handy when you're thick into the mix and want to minimize mouse movements. Definitely inspires me to F things up.
When jamming with qwerty, chances are that you are using your mouse to navigate and adjust. If you happen to be somewhere with a lot of sunlight you could get a devious glare on your screen and the first thing you lose is your mouse arrow (second thing being your mind!!!!). Something that helps make it easier to spot in a frenzy is changing your mouse settings so that your cursor is massive!
I enjoy playing notes or drums using the asdf row, and when it is activated it overrides key assignments. The black keys are up for grabs but my clumsy fingers still prefer to avoid accidents...
My mouse has extra buttons on the sides. The software lets me set one to be 'slower movements' for finer detail. The other button I've set to ctrl, then I can do ctrl functions using one finger

This one I take for granted, Spacebar to start and stop your tracks!!! If you don't know this, practice it until your household runs out of chairs that are musical. (and then get acquainted with shift+spacebar!)
If anyone has any tips, no matter how obvious or silly, please do share
